Optimizing liquid storage duration of two poultry species semen with plant based extender
Semen preservation in poultry
Keywords:Semen preservation, Plant-based extender, Semen quality, Turkey, Chicken
Liquid storage of semen preservation for poultry species is deemed to be the only reliable and cost effective method compare to cryopreservation. This experiment was conducted to examine the preservation capacity of Tris coconut-water orange juice extender (TCWOE) on tom and cock semen for artificial insemination. TCWOE was prepared. Semen ejaculates were collected and pooled from five toms and cocks separately. The pooled semen from each species was divided into two portions and in one portion of each species the extender was added in the ratio of 1:3 (semen: extender), making a total of four treatments. Experimental design used was a complete randomized design consisting of three trials. Semen microscopic parameters like motility, viability, membrane integrity, and acrosome integrity were examined and recorded for freshly extended semen and preserved semen at 4-8oC every 12 h interval till 72 h. The motility and membrane integrity of freshly extended cock semen was significantly higher (P<0.05) compared to motility of extended tom semen and membrane integrity of un-extended tom semen. During storage up to 24 h, extended tom and cock semen showed significantly higher (P<0.05) motility and membrane integrity compare to un-extended semen. Extended cock semen had significant (P<0.05) higher percentage motility, livability and membrane integrity compared to un-extended and extended tom semen from 36 h to 72 h of storage. However, no significant difference (P>0.05) was observed for acrosome integrity. Conclusively, cock semen survived longer and had better results considerable for artificial insemination than tom semen up to 72 h storage. However, the extender was beneficial for storage of both species semen.
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