Integrated approaches to the diagnosis, molecular characterisation, epidemiological surveillance, and prevention of pasteurellosis in farm animals

Integrated approaches to pasteurellosis management

Authors

  • Assiya Mussayeva LLP Kazakh Scientific-Research Veterinary Institute, Almaty, Republic of Kazakhstan
  • Sholpan Baramova LLP Kazakh Scientific-Research Veterinary Institute, Almaty, Republic of Kazakhstan
  • Batyrbek Aitzhanov LLP Kazakh Scientific-Research Veterinary Institute, Almaty, Republic of Kazakhstan https://orcid.org/0009-0004-2985-1412
  • Alim Bizhanov LLP Kazakh Scientific-Research Veterinary Institute, Almaty, Republic of Kazakhstan
  • Kuandyk Shynybayev LLP Kazakh Scientific-Research Veterinary Institute, Almaty, Republic of Kazakhstan

DOI:

https://doi.org/10.62310/liab.v6i1.429

Keywords:

Genetic typing, Territorial zoning, Cattle, Infectious pathologies, Microclimate parameters

Abstract

The aim of this study was to summarise and analyse contemporary diagnostic and preventive measures to improve the effectiveness of epizootiological monitoring of bacterial infection. The review is based on an analysis of publications on methods of clinical examination, bacteriological isolation, serological diagnosis, polymerase chain reaction, molecular typing, and antibiotic susceptibility testing. The analysis showed that integrating molecular diagnostics, serological surveillance, epizootiological assessment, and farm-level risk assessments is necessary for effective pasteurellosis control. The analysis showed that variations in livestock management practices, environmental factors, and veterinary surveillance systems are associated with regional differences in occurrence and transmission of disease. The study found that while territorial analysis enables the evaluation of infection distribution and the identification of regions in need of increased surveillance, genetic identification techniques offer useful information on pathogen features. The results reveal that production and organisational elements that affect the development of disease should be taken into account in conjunction with diagnostic procedures. To improve preventive measures against pasteurellosis, an integrated surveillance strategy that combines laboratory confirmation, genetic characterisation, environmental monitoring, and veterinary control measures was developed. The findings offer a foundation for improving management of bacterial illnesses in farm animals and tailoring diagnostic and preventive programs to local epizootiological conditions. The practical value of the study lies in the possibility of applying the obtained findings by veterinary services, farms, and laboratories to improve the diagnosis, epizootiological monitoring, and prevention of pasteurellosis, taking into account regional characteristics.

Metrics

Metrics Loading ...

References

Abbas A, Khan RT, Asghar AY, Shafeeq M, Rahim A. (2023). Pneumonic Pasteurellosis: Role of Pasteurella multocida and Mannheimia haemolytica in respiratory disease of cattle. Research Journal of Agricultural Sciences 2(1): 94-110. https://doi.org/10.5281/zenodo.10072315

Abera D, Mossie T. (2023). A review on pneumonic pasteurellosis in small ruminants. Journal of Applied Animal Research 51(1): 1-10. https://doi.org/10.1080/09712119.2022.2146123

Absatirov G, Smagulov D, Bozymov K, Shalmenov M, Nassambayev Y, Yessengaliyev K, Baitlessova L, Girisgin AO. (2025). Risks to the growth, conservation and management of the Ural saiga population. Diversity 17(9): 595. https://doi.org/10.3390/d17090595

Alemu SA, Belachew YD, Tefera TA. (2023). Isolation and molecular detection of Mannheimia haemolytica and Pasteurella multocida from clinically pneumonic pasteurellosis cases of Bonga sheep breed and their antibiotic susceptibility tests in selected areas of southwest Ethiopian people’s regional state, Ethiopia. Veterinary Medicine: Research and Reports 14: 233-244. https://doi.org/10.2147/VMRR.S435932

Alexyuk M, Bogoyavlenskiy A, Alexyuk P, Moldakhanov Y, Berezin V, Digel I. (2021). Epipelagic microbiome of the Small Aral Sea: Metagenomic structure and ecological diversity. MicrobiologyOpen 10(1): e1142. https://doi.org/10.1002/mbo3.1142

Ali H, Waseem M, Khaliq A, Salman M, Saad MA, Bashir MA, Ishaq M, Hameed A. (2025). Pasteurella multocida in veterinary medicine emerging resistance, vaccination challenges, and control approaches. Haya: The Saudi Journal of Life Sciences 10(8): 350-361. https://doi.org/10.36348/sjls.2025.v10i08.010

Ali S, Tariq MH, Yaqoob M, Haq MU, Zahra R. (2025). Molecular epidemiology and characterization of antibiotic resistance of Pasteurella multocida isolated from livestock population of Punjab, Pakistan. International Journal of Veterinary Science and Medicine 13(1): 1-12. https://doi.org/10.1080/23144599.2024.2437223

Asfaw M, Senbit M, Yesuf M, Dagnaw M, Birhan G, Abat AS, Ibrahim SM. (2022). A preliminary investigation on a commercial ovine pasteurellosis vaccine using clinical and pathological endpoints. Infection and Drug Resistance 15: 2937-2948. https://doi.org/10.2147/IDR.S365745

Auganov AN, Tynysbekov BK, Akhmetollaev IA. (2024). Importance of identification of amino acid substitutions for detection of Pasteurella multocida belonging to a serogroup. Biobezopasnost i Biotekhnologiya 20: 14-28. https://doi.org/10.58318/2957-5702-2024-20-14-28

Barta L, Stöger A, Polzer D, Ruppitsch W, Schmoll F, Sattler T. (2025). Phenotypical and genotypical resistance testing of Pasteurella multocida isolated from different animal species in Austria. Frontiers in Veterinary Science 12: 1640536. https://doi.org/10.3389/fvets.2025.1640536

Bitew Z, Abayneh Tefera T, Deneke Y, Tmariam T, Yihunie FB. (2025). Molecular serotyping and antimicrobial susceptibility profiles of Pasteurella multocida isolated from cases of hemorrhagic septicemia in cattle from selected districts of Keffa and Bench Sheko zones, South West Ethiopia. BMC Microbiology 25(1): 224. https://doi.org/10.1186/s12866-025-03947-z

Bondarenko G, Nosevych D, Kruk O, Chumachenko I. (2023). Technological solutions for effective production on beef cattle breeding farms in the conditions of Ukraine. Animal Science and Food Technology 14(4): 40–57. https://doi.org/10.31548/animal.4.2023.40

Buienbayeva ZK, Latypova ZA, Issakulova BZ, Bakiyeva FA, Namet AM, Valdovska A. (2024). Development of veterinary and sanitary measures for the prevention of pasteurellosis infection in cattle: The case of the Republic of Kazakhstan. International Journal of Veterinary Science 13(2): 164-171. https://doi.org/10.47278/journal.ijvs/2023.085

Bulashev A, Eskendirova S, Ingirbay B, Syzdykova A, Zhagipar F. (2023). Validation of a chimeric proteins-based ELISA for the diagnosis of brucellosis in Kazakhstan. Turkish Journal of Veterinary & Animal Sciences 47(6): 588-597. https://doi.org/10.55730/1300-0128.4329

Casalino G, D’Amico F, Bozzo G, Dinardo FR, Schiavitto M, Galante D, Aceti A, Ceci E, Romito D, D’Onghia F, Dimuccio M, Camarda A, Circella E. (2024). In field evaluation of impact on clinical signs of an inactivated autogenous vaccine against Pasteurella multocida in rabbits. International Journal of Veterinary Science and Medicine 12(1): 39-47. https://doi.org/10.1080/23144599.2024.2348900

Domínguez-Odio A, Delgado DL. (2023). Global commercialization and research of veterinary vaccines against Pasteurella multocida: 2015-2022 technological surveillance. Veterinary World 16(5): 946. https://doi.org/10.14202/vetworld.2023.946-956

Ducrocq C, Luc F, Zeggay A, Petit M, Ould-Slimane M. (2023). Unusual presentation of lumbar surgical site infection: A case study of early onset Pasteurella multocida Infection after Arthrodesis. The American Journal of Case Reports 24: e941844-1. https://doi.org/10.12659/AJCR.941844

Džermeikaitė K, Bačėninaitė D, Antanaitis R. (2023). Innovations in cattle farming: Application of innovative technologies and sensors in the diagnosis of diseases. Animals 13(5): 780. https://doi.org/10.3390/ani13050780

Fegan JE, Waeckerlin RC, Tesfaw L, Islam EA, Deresse G, Dufera D, Assefa E, Gray-Owen SD. (2024). Developing a PmSLP3-based vaccine formulation that provides robust long-lasting protection against hemorrhagic septicemia – Causing serogroup B and E strains of Pasteurella multocida in cattle. Frontiers in Immunology 15: 1392681. https://doi.org/10.3389/fimmu.2024.1392681

Gabdullin D, Julanov M, Tagayev O, Zainettinova D, Kadraliyeva B, Zakirova F, Kozhayeva A, Sidikhov B, Zholdasbekov A, Yertleuova B, Aitpayeva Z. (2026). Evaluation of blood parameter changes on treatment efficacy in cows with purulent-catarrhal endometritis in farms of West Kazakhstan Region. International Journal of Veterinary Science 15(1): 225–232. https://doi.org/10.47278/journal.ijvs/2025.113

Ginayatov N, Aitpayeva Z, Zhubantayev I, Kassymbekova L, Zhanabayev A, Abulgazimova G, et al. (2025). Smallholder Cattle Farmers’ Knowledge, Attitudes, and Practices Toward Rabies: A Regional Survey in Kazakhstan. Veterinary Sciences 12(4): 335. https://doi.org/10.3390/vetsci12040335

Heshteli RR, Paimard G, Adabi M, Esmaeili S. (2025). Advances in biosensors: A breakthrough in rapid and precise brucellosis detection. Analytical Biochemistry 700: 115782. https://doi.org/10.1016/j.ab.2025.115782

Irgashev A, Nurgaziev R, Nurmanov C, Asanova E, Ishenbaeva S. (2023). Respiratory form of infectious rhinotracheitis: Analysis of immunomorphological reactions. Scientific Horizons 26(10): 33–43. https://doi.org/10.48077/scihor10.2023.32

Ishchanova AS, Kozhayeva AR, Yertleuova BO, Gabdullin DY. (2025). Analysis of the epizootic dynamics of registered infectious diseases in the West Kazakhstan region. Science and Education 4(81): 3-10. https://doi.org/10.52578/2305-9397-2025-4-1-3-10

Jahromi FM, Tahamtan Y, Kargar M, Doosti A, Kafilzadeh F. (2025). Molecular evaluation of Pasteurella multocida in livestock, characterization of virulence genes, and pathogenic potential of the retrieved isolates in Iran. Iranian Journal of Veterinary Research 26(2): 107. https://doi.org/10.22099/ijvr.2025.51317.7617

Khussainov D, Mukhatayeva K, Ashirova Z, Yessimsiitova Z, Mutushev A, Idayat N, Davletova A, Karbozova A. (2024). Epizootic measures against infectious and viral animal diseases in Kazakhstan. Caspian Journal of Environmental Sciences 22(5): 1243-1253. https://doi.org/10.22124/CJES.2024.8340

Kozhayeva A, Kereyev A, Shevtsov A, Abdigulov B, Smagulov D, Khamzina S, Kulzhanova B, Nurzhanova K, Bupebayeva L, Khamzina A. (2025). Molecular prevalence of Coenurus cerebralis in sheep exhibiting neurological symptoms in Kazakhstan. Frontiers in Veterinary Science 12: 1620425. https://doi.org/10.3389/fvets.2025.1620425

Kumar M, Fofaria N, Roy A. (2025). Haemorrhagic septicemia in bovines: Its diagnosis and therapeutic management. Bio Vet Innovator 2(6): 12-19. https://doi.org/10.5281/zenodo.15624158

Kushaliev K, Kozhayeva A, Sadenov M, Khayrushev A. (2022). Helminthoses of saigas in their migration territory. Science and Education 1(66): 60-71. https://doi.org/10.52578/2305-9397-2022-1-1-60-70

Kushaliyev K, Shamshidin A, Kozhayeva A, Alzhappar M, Tleulenov Z. (2024). Creation of nurseries and veterinary preventive measures for saigas of Betpakdala and Ural populations in Kazakhstan. Scientific Horizons 27(1): 41-53. https://doi.org/10.48077/scihor1.2024.41

Kutumbetov L, Myrzakhmetova B, Tussipova A, Zhapparova G, Bissenbayeva K, Tlenchiyeva T, Nurabayev S, Kerimbayev A, Orynbayev M, Makhashov E, Zhugunissov K. (2025). Susceptibility and transmission dynamics of peste des petits ruminants virus in domestic and wild ruminants: Experimental insights from Kazakhstan. Viruses 17(9): 1231. https://doi.org/10.3390/v17091231

Lachowicz-Wolak A, Chmielina A, Przychodniak I, Karwańska M, Siedlecka M, Klimowicz-Bodys M, Dyba K, Rypuła K. (2025). Antimicrobial-resistance and virulence-associated genes of Pasteurella multocida and Mannheimia haemolytica isolated from polish dairy calves with symptoms of bovine respiratory disease. Microorganisms 13(3): 491. https://doi.org/10.3390/microorganisms13030491

Laganà P, Votano L, Caruso G, Azzaro M, Lo Giudice A, Delia S. (2018). Bacterial isolates from the Arctic region (Pasvik River, Norway): Assessment of biofilm production and antibiotic susceptibility profiles. Environmental Science and Pollution Research 25(2): 1089–1102. https://doi.org/10.1007/s11356-017-0485-1

Latypova ZA, Namet AM, Isakulova BZh, Buienbaeva ZK, Kerimbaeva RA. (2022). Zoning of the territory of the Republic of Kazakhstan by the degree of spread of pasteurellosis infection among cattle. Microbiology and Virology 3(38): 55-63. https://imv-journal.kz/index.php/mav/article/view/56

Lloyd JB. (2025). Survey of Mycoplasma (Mesomycoplasma) ovipneumoniae, Mannheimia haemolytica, and Pasteurella multocida in pneumonic lungs from sheep slaughtered at 3 abattoirs in New South Wales, Australia. Canadian Journal of Veterinary Research 89(2): 83-87. https://www.ingentaconnect.com/content/cvma/cjvr/2025/00000089/00000002/art00004

Megahed MM, El-Nagar AM, El-Demerdash AS, Ayoub MA, Tolba HM. (2023). Evaluation and development of diagnostic tools for rapid detection of Riemerella anatipestifer and Pasteurella multocida in ducks. Journal of Advanced Veterinary and Animal Research 10(2): 211-221. https://doi.org/10.5455/javar.2023.j671

Mekonnen Y. (2024). A review on ovine Pasteurellosis. World Journal of Veterinary Science 4: 1023. https://www.medtextpublications.com/open-access/a-review-on-ovine-pasteurellosis-1668.pdf

Molaee H, Tahamtan Y, Saeednezhad E, Hayati M. (2022). Isolation of the various serotypes of Mannheimia haemolytica and preparation of the first vaccine candidate in Iran. Molecular Biology Reports 49(11): 10367-10375. https://doi.org/10.1007/s11033-022-07890-4

Mukhamadieva N, Julanov M, Zainettinova D, Stefanik V, Nurzhumanova Z, Mukataev A, Suychinov A. (2022). Prevalence, diagnosis and improving the effectiveness of therapy of mastitis in cows of dairy farms in East Kazakhstan. Veterinary Sciences 9(8): 398. https://doi.org/10.3390/vetsci9080398

Mutushev NI, Davletova A, Karbozova A. (2024). Epizootic measures against infectious and viral animal diseases in Kazakhstan. Caspian Journal of Environmental Sciences 22(5): 1243-1253. https://doi.org/10.22124/CJES.2024.8340

Okella H, Tonooka K, Okello, E. (2023). A systematic review of the recent techniques commonly used in the diagnosis of Mycoplasma bovis in dairy cattle. Pathogens 12(9): 1178. https://doi.org/10.3390/pathogens12091178

Orkara S, Khamzina A, Sandybayev N, Sattarova R, Davletova A, Khamzin K, Pokharel K, Ibraginov P, Burambayeva N, Smagulov D. (2025). Epidemiological landscape and genetic prospects for marker-assisted selection in Kazakh sheep. Frontiers in Veterinary Science 12: 1647959. https://doi.org/10.3389/fvets.2025.1647959

Poussard M, Pant SD, Huang J, Scott P, Ghorashi SA. (2025). Comparative evaluation of PCR and loop-mediated isothermal amplification (LAMP) assays for detecting Pasteurella multocida in poultry. New Zealand Veterinary Journal 73(2): 134-142. https://doi.org/10.1080/00480169.2024.2417921

Puig A, Ruiz M, Bassols M, Fraile L, Armengol R. (2022). Technological tools for the early detection of bovine respiratory disease in farms. Animals 12(19): 2623. https://doi.org/10.3390/ani12192623

Rahman MA, Zereen F, Hossain MG, Al-Amin M, Alam J, Shimada M, Ragman T, Saha S. (2024). The first report of concurrent infection of hemorrhagic septicemia with foot and mouth disease in cattle in Bangladesh. Comparative Immunology, Microbiology and Infectious Diseases 115: 102272. https://doi.org/10.1016/j.cimid.2024.102272

Ribeiro MG, da Silva Ribeiro AB, da Silva AB, Mariano GH, de Sá Teles Bertunes L, Portilho FV, Paes AC. (2024). Peritonitis-related bacterial infections: A large-scale case-series retrospective study in 160 domestic animals (2009-2022). Brazilian Journal of Microbiology 55(4): 4205-4217. https://doi.org/10.1007/s42770-024-01551-3

Robi DT, Mossie T, Temteme S. (2024). A comprehensive review of the common bacterial infections in dairy calves and advanced strategies for health management. Veterinary Medicine: Research and Reports 15: 1-14. https://doi.org/10.2147/VMRR.S452925

Sabyrova A, Duyssembayev S, Koigeldinova A, Kozhayeva A, Suleimenov S, Zainettinova D, Temirova A, Zhakiyanova MS, Akhmadiyeva A. (2026). Methods for optimizing microclimate parameters in barns for cattle in Kazakhstan. International Journal of Veterinary Science 15(2): 591–598. https://doi.org/10.47278/journal.ijvs/2025.175

Shakibayev Ye, Kydyrova G, Bojko G, Orynbaeva B, Kadyrkulova M. (2026). Epizootiological situation of brucellosis of cattle and small ruminants in the Almaty region of the Republic of Kazakhstan. Bulletin of the Kyrgyz National Agrarian University 24(2): 19–29. https://doi.org/10.63621/bknau./2.2026.19

Storoni C, Preziuso S, Attili AR, Li Y, Cuteri V. (2025). Bacterial bovine respiratory disease: A comprehensive review of etiology, pathogenesis, and management strategies. Microbiology Research 17(1): 18. https://doi.org/10.3390/microbiolres17010018

Szacawa E, Radulski Ł, Weiner M, Szulowski K, Krajewska-Wędzina M. (2025). Mycobacterium tuberculosis complex infections in animals: A comprehensive review of species distribution and laboratory diagnostic methods. Pathogens 14(10): 1004. https://doi.org/10.3390/pathogens14101004

Tabatabaei M, Abdolahi F. (2023). Molecular evaluation of sheep and goats isolates of Pasteurella multocida and their antibiotic resistance. Veterinary Research Forum 14(9): 481-487. https://doi.org/10.30466/vrf.2022.556438.3524

Tagaev O, Aitpayeva Z, Batyrbekov A, Shektibayev M. (2025). Influence of microclimate parameters depending on ownership type on the condition of dairy cows. 3i: Intellect, Idea, Innovation 4: 30-36. https://doi.org/10.52269/22266070_2024_4_30

Umuraliyev BK, Seisenbayeva MS, Zhakypbek AS, Rsaliev AS, Isakhan AA, Orazymbetova NK, Abduraimov EO. (2025). Production of hyperimmune serum against anthrax. Eurasian Journal of Applied Biotechnology 4: 95-102. https://doi.org/10.11134/btp.4.2025.11

Van Nguyen T, Trinh TH, Van Nguyen T, Ng-Nguyen D, Nguyen HQ, Vu-Khac H. (2025). Comprehensive capsular, lipopolysaccharide, virulence, and antimicrobial resistance profiling of Pasteurella multocida isolated from buffaloes in Vietnam: First report of capsular type D and predominant L2 genotype. Veterinary World 18(12): 4069-4081. https://doi.org/10.14202/vetworld.2025.4069-4081

Verzhykhovsky O, Nedosekov V. (2024). Key aspects of biosafety in modern animal husbandry. Ukrainian Journal of Veterinary Sciences 15(3): 41–54. https://doi.org/10.31548/veterinary3.2024.41

Vygovska L, Bhattacharya Ch, Ushkalov V, Vishovan Yu, Danchuk V. (2023). Antibiotic resistance of microorganisms isolated from cows with subclinical mastitis. Ukrainian Journal of Veterinary Sciences 14(2): 28–42. https://doi.org/10.31548/veterinary2.2023.28

Wentzel JM, Biggs LJ, Van Vuuren M. (2022). Comparing the minimum inhibitory and mutant prevention concentrations of selected antibiotics against animal isolates of Pasteurella multocida and Salmonella typhimurium. Onderstepoort Journal of Veterinary Research 89(1): 1955. https://journals.co.za/doi/abs/10.4102/ojvr.v89i1.1955

Zhaksylyk S, Sultankulova K, Boronbaeva A, Shynybekova G, Kozhabergenov N. (2026). Optimisation of PCR conditions for the detection of infectious bovine rhinotracheitis. Bulletin of the Kyrgyz National Agrarian University 24(1): 10–17. https://doi.org/10.63621/bknau./1.2026.10

Zhao M, Wu W, Song W, Yang H, Liu H, Xie R, Huang X, Huang J, Hau L, Chen H, Wu B, Peng Z. (2025). A quadruplex real-time fluorescent quantitative PCR detection method for identification and serotyping of Pasteurella multocida. Microbial Pathogenesis 207: 107889. https://doi.org/10.1016/j.micpath.2025.107889

Downloads

Published

11-09-2026

How to Cite

Mussayeva, A., Baramova, S., Aitzhanov, B., Bizhanov, A., & Shynybayev, K. (2026). Integrated approaches to the diagnosis, molecular characterisation, epidemiological surveillance, and prevention of pasteurellosis in farm animals: Integrated approaches to pasteurellosis management. Letters In Animal Biology, 6(1), 166–176. https://doi.org/10.62310/liab.v6i1.429

Issue

Section

Review Articles
Recieved 2026-08-17
Accepted 2026-09-04
Published 2026-09-11